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lenticrispr v2 blast vector  (Addgene inc)


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    Structured Review

    Addgene inc lenticrispr v2 blast vector
    Lenticrispr V2 Blast Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenticrispr+v2+blast+vector/lentiCRISPR+v2-Blast+(Plasmid+%2383480)/pmc12872570-89-8-12
    Average 96 stars, based on 230 article reviews
    lenticrispr v2 blast vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Profiling triple-negative breast cancer-specific super-enhancers identifies high-risk mesenchymal development subtype and BETi-Targetable vulnerabilities.
    Article Snippet: All cell lines from ATCC were authenticated using short tandem repeat (STR) profiling, passaged for fewer than 10 passages, and routinely tested for Mycoplasma contamination. .. The gRNA targeting the SE proximal site was duplexed and cloned into the lentiCRISPR v2-puro vector (Addgene Plasmid #98290), while the gRNA targeting the SE distal site was cloned into the lentiCRISPR v2-blast vector (Addgene Plasmid #83480). .. The gRNA targeting the SE proximal site was duplexed and cloned into the lentiCRISPR v2-puro vector (Addgene Plasmid #98290), while the gRNA targeting the SE distal site was cloned into the lentiCRISPR v2-blast vector (Addgene Plasmid #83480).

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels and regulates cGAMP transport.
    Article Snippet: .. For hard-to-transfect cells (i.e., Jurkat and TIME cells), the same guide RNA was cloned into lentiCRISPR-v2-Blast vector (Addgene). .. Lentiviruses were produced by co-transfecting it with packaging vectors (pVSV-G, pMDL, and pRSV) in HEK293 cells following Addgene’s instruction.

    Article Title: Profiling triple-negative breast cancer-specific super-enhancers identifies high-risk mesenchymal development subtype and BETi-Targetable vulnerabilities
    Article Snippet: .. The gRNA targeting the SE proximal site was duplexed and cloned into the lentiCRISPR v2-puro vector (Addgene Plasmid #98290), while the gRNA targeting the SE distal site was cloned into the lentiCRISPR v2-blast vector (Addgene Plasmid #83480). ..

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels and regulates cGAMP transport
    Article Snippet: .. For hard-to-transfect cells (i.e., Jurkat and TIME cells), the same guide RNA was cloned into lentiCRISPR-v2-Blast vector (Addgene). .. Lentiviruses were produced by co-transfecting it with packaging vectors (pVSV-G, pMDL, and pRSV) in HEK293 cells following Addgene’s instruction.

    Article Title: Endogenous CD5L controls the metabolic and inflammatory state of human macrophages
    Article Snippet: .. CD5L-targeting oligonucleotides were synthesized and cloned into the lentiCRISPR v2-Blast vector (83480, Addgene) individually, and three validated targeting clones were used to generate the DKO mutant as described before ( ). .. Total cellular RNA was isolated by RNeasy Mini kit (74106) with DNAse I digestion (79254) using the QIAcube instrument (all from Qiagen) according to the manufacturer’s instructions.

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels
    Article Snippet: .. For hard-to-transfect cells (i.e., Jurkat and TIME cells), the same guide RNA was cloned into lentiCRISPR-v2-Blast vector (Addgene). .. Lentiviruses were produced by co-transfecting it with packaging vectors (pVSV-G, pMDL, and pRSV) in HEK293 cells following Addgene’s instruction.

    Plasmid Preparation:

    Article Title: Profiling triple-negative breast cancer-specific super-enhancers identifies high-risk mesenchymal development subtype and BETi-Targetable vulnerabilities.
    Article Snippet: All cell lines from ATCC were authenticated using short tandem repeat (STR) profiling, passaged for fewer than 10 passages, and routinely tested for Mycoplasma contamination. .. The gRNA targeting the SE proximal site was duplexed and cloned into the lentiCRISPR v2-puro vector (Addgene Plasmid #98290), while the gRNA targeting the SE distal site was cloned into the lentiCRISPR v2-blast vector (Addgene Plasmid #83480). .. The gRNA targeting the SE proximal site was duplexed and cloned into the lentiCRISPR v2-puro vector (Addgene Plasmid #98290), while the gRNA targeting the SE distal site was cloned into the lentiCRISPR v2-blast vector (Addgene Plasmid #83480).

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels and regulates cGAMP transport.
    Article Snippet: .. For hard-to-transfect cells (i.e., Jurkat and TIME cells), the same guide RNA was cloned into lentiCRISPR-v2-Blast vector (Addgene). .. Lentiviruses were produced by co-transfecting it with packaging vectors (pVSV-G, pMDL, and pRSV) in HEK293 cells following Addgene’s instruction.

    Article Title: Profiling triple-negative breast cancer-specific super-enhancers identifies high-risk mesenchymal development subtype and BETi-Targetable vulnerabilities
    Article Snippet: .. The gRNA targeting the SE proximal site was duplexed and cloned into the lentiCRISPR v2-puro vector (Addgene Plasmid #98290), while the gRNA targeting the SE distal site was cloned into the lentiCRISPR v2-blast vector (Addgene Plasmid #83480). ..

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels and regulates cGAMP transport
    Article Snippet: .. For hard-to-transfect cells (i.e., Jurkat and TIME cells), the same guide RNA was cloned into lentiCRISPR-v2-Blast vector (Addgene). .. Lentiviruses were produced by co-transfecting it with packaging vectors (pVSV-G, pMDL, and pRSV) in HEK293 cells following Addgene’s instruction.

    Article Title: Endogenous CD5L controls the metabolic and inflammatory state of human macrophages
    Article Snippet: .. CD5L-targeting oligonucleotides were synthesized and cloned into the lentiCRISPR v2-Blast vector (83480, Addgene) individually, and three validated targeting clones were used to generate the DKO mutant as described before ( ). .. Total cellular RNA was isolated by RNeasy Mini kit (74106) with DNAse I digestion (79254) using the QIAcube instrument (all from Qiagen) according to the manufacturer’s instructions.

    Article Title: ABCC4 impairs the clearance of plasma LDL cholesterol through suppressing LDLR expression in the liver
    Article Snippet: .. Each pair of oligonucleotides were annealed and ligated into the lentiCRISPR v2-Blast vector (Addgene#83480). ..

    Article Title: ABCC4 impairs the clearance of plasma LDL cholesterol through suppressing LDLR expression in the liver.
    Article Snippet: .. Each pair of oligonucleotides were annealed and ligated into the lentiCRISPR v2-Blast vector (Addgene#83480). ..

    Article Title: Puromycin-sensitive aminopeptidase acts as an inhibitory auxiliary subunit of volume-regulated anion channels
    Article Snippet: .. For hard-to-transfect cells (i.e., Jurkat and TIME cells), the same guide RNA was cloned into lentiCRISPR-v2-Blast vector (Addgene). .. Lentiviruses were produced by co-transfecting it with packaging vectors (pVSV-G, pMDL, and pRSV) in HEK293 cells following Addgene’s instruction.

    Synthesized:

    Article Title: Endogenous CD5L controls the metabolic and inflammatory state of human macrophages
    Article Snippet: .. CD5L-targeting oligonucleotides were synthesized and cloned into the lentiCRISPR v2-Blast vector (83480, Addgene) individually, and three validated targeting clones were used to generate the DKO mutant as described before ( ). .. Total cellular RNA was isolated by RNeasy Mini kit (74106) with DNAse I digestion (79254) using the QIAcube instrument (all from Qiagen) according to the manufacturer’s instructions.

    Mutagenesis:

    Article Title: Endogenous CD5L controls the metabolic and inflammatory state of human macrophages
    Article Snippet: .. CD5L-targeting oligonucleotides were synthesized and cloned into the lentiCRISPR v2-Blast vector (83480, Addgene) individually, and three validated targeting clones were used to generate the DKO mutant as described before ( ). .. Total cellular RNA was isolated by RNeasy Mini kit (74106) with DNAse I digestion (79254) using the QIAcube instrument (all from Qiagen) according to the manufacturer’s instructions.



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    Image Search Results


    A : Representative immunoblots of n = 3 experiments done using total lysates from BLM wild-type (Wt), BLM empty vector (EV)-infected, or two different CRISPR/ Cas9 -mediated MEK5 k.o. single cell clones (SCC) of BLM treated with diluent (ctrl) or 25 nM Tram for 14 days, showing expression of selected cell cycle proteins. ERK5 phosphorylation and DUSP4 suppression confirm Tram functionality. Successful MEK5 gene disruption was analysed by MEK5 immunoblot and functionally evaluated by immunoblotting for ERK5, which confirmed absent ERK5 autophosphorylation. Tubulin served as loading control. B : Representative cell cycle profiles of n = 2 experiments, as determined by flow cytometric analysis of the indicated PI-stained conditions with percentages of S-phase cells indicated. C : Cell doubling time analysis representative of n = 2 experiments with running times of 2–5 weeks performed with Wt BLM or the indicated MEK5 k.o. BLM SCCs cultured in absence or presence of Tram. The shown experiment run over a period of five weeks.

    Journal: Cell Death & Disease

    Article Title: MEK5/ERK5 inhibition sensitizes NRAS -mutant melanoma to MAPK-targeted therapy by preventing Cyclin D/CDK4-mediated G1/S progression

    doi: 10.1038/s41419-025-08036-7

    Figure Lengend Snippet: A : Representative immunoblots of n = 3 experiments done using total lysates from BLM wild-type (Wt), BLM empty vector (EV)-infected, or two different CRISPR/ Cas9 -mediated MEK5 k.o. single cell clones (SCC) of BLM treated with diluent (ctrl) or 25 nM Tram for 14 days, showing expression of selected cell cycle proteins. ERK5 phosphorylation and DUSP4 suppression confirm Tram functionality. Successful MEK5 gene disruption was analysed by MEK5 immunoblot and functionally evaluated by immunoblotting for ERK5, which confirmed absent ERK5 autophosphorylation. Tubulin served as loading control. B : Representative cell cycle profiles of n = 2 experiments, as determined by flow cytometric analysis of the indicated PI-stained conditions with percentages of S-phase cells indicated. C : Cell doubling time analysis representative of n = 2 experiments with running times of 2–5 weeks performed with Wt BLM or the indicated MEK5 k.o. BLM SCCs cultured in absence or presence of Tram. The shown experiment run over a period of five weeks.

    Article Snippet: BLM, lentiCRISPR_zeo, a derivative of the lentiviral dual gRNA/ Cas9 expression vector lentiCRISPRv2-Blast (Addgene_83480) replacing the blasticidin R gene by a zeocin R gene was used.

    Techniques: Western Blot, Plasmid Preparation, Infection, CRISPR, Clone Assay, Expressing, Phospho-proteomics, Disruption, Control, Staining, Cell Culture